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4.2.2 Mechanism of action in dry eye disease LFs antioxidant properties on the ocular surface, where it chelates free iron and prevents the formation of harmful hydroxyl radicals, thus protecting CECs from oxidative stress (Pastori et al., 2015)

Heavy NNMT activity drains the methyl group supply, affecting all those downstream methylation reactions

Warming vials to speed dissolution risks heat damage

Deficiencies in glutathione reductase can lead to a decrease in the levels of active glutathione, which may result in: Increased susceptibility to oxidative stress and cellular damage

For example, E50K mutated OPTN induced impairment of autophagy that led to cell death in RGC-5 cell lines 28 , and rapamycin, an autophagic inducer, protected RGCs in E50K OPTN overexpressed rats in vivo 29