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After 96 h, the cells were harvested using pancreatic enzymes, washed twice with PBS, and lysed with 1% TritonX-100 (300 L for 0 M CGA and 150 L for 600 M CGA) on ice for 10 min

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As we age, they can be more likely to suffer from adult-onset growth hormone deficiency (AGHD), characterized by a relative increase in fat mass, and a decrease in muscle mass